Area(s) of Expertise
- Analytical Chemistry, Biochemistry, Proteomics and Mass Spectrometry
Prof. Ivanov earned his Ph.D. in Bioorganic Chemistry at the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow in 2000. He initially joined Northeastern University as a postdoctoral fellow at the Barnett Institute (2000 – 2003). Then he joined Harvard University first as Assistant Director and then he was promoted to Associate Director of the Harvard NIEHS Center for Environmental Health Proteomics Facility at the Department of Genetics and Complex Diseases (GCD) of the Harvard School of Public Health (HSPH). Dr. Ivanov continued his tenure at Harvard University becoming Director of the HSPH Proteomics Resource in 2008. Dr. Ivanov re-joined Northeastern as Research Associate Professor at the Barnett Institute of Chemical and Biological Analysis in 2011. In 2017, he accepted a position of an Associate Professor at the Department of Chemistry & Chemical Biology and Faculty Fellow at the Barnett Institute of Chemical & Biological Analysis.
Prof. Ivanov’s research focuses on the development of microscale liquid phase separation- and mass spectrometry (MS)-based proteomic technologies for solving challenging biomedical problems. The areas he is currently working on include: (1) deep proteomic profiling of limited biological samples (e.g., rare circulating cells, microdissected cells from tissue microscale biospecimens) using ultra-low flow liquid phase separation techniques, including capillary electrophoresis (CE) and ultra-narrow bore column liquid chromatography (LC) interfaced with advanced MS; (2) comprehensive characterization of protein isoforms, modifications (e.g. post-translational modifications, chemical modifications, sequence variants, charge variants), and protein complexes (in their native non-denatured states), including characterization of biopharmaceuticals, using a combination of bottom-up, middle-down, top-down and native proteomic approaches; (3) development of ultra-low flow liquid phase separation techniques, including CE and ultra-narrow monolithic and porous layer open tubular column LC for high sensitivity (attomole and low zeptomole level) MS analysis; and (4) isolation and characterization of extracellular vesicles, including circulating exosomes and microparticles, as potential sources of biomarkers for disease diagnostics and therapy delivery vehicles. He has been actively involved in national and international initiatives enabled under the umbrella of the ABRF to develop standards for proteomics research and standardize proteomic practices. Dr. Ivanov’s expertise involves advanced sample preparation, liquid phase separation, MS and bioinformatics techniques.